CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.

Ran PengYe WangWan-Wan FengXin-Jing YueJiang-He ChenXiao-Zhuang HuZhi-Feng LiDuo-Hong ShengYou-Ming ZhangYue-Zhong Li

Published in: Microbial cell factories (2018)

Our results showed that the CRISPR/dCas-mediated transcription activation is a simple and broadly applicable technique to improve the transcriptional efficiency for the production of secondary metabolites in microorganisms. This is the first time to construct the CRISPR/dCas9 activation system in myxobacteria and the first time to assay the CRISPR/dCas9 activations for the biosynthesis of microbial secondary metabolites.

Keyphrases

genome wide
genome editing
crispr cas
dna methylation
transcription factor
ms ms
gene expression
high throughput
heat shock
microbial community
oxidative stress
heat stress