ISSLS prize in basic science 2021: a novel inducible system to regulate transgene expression of TIMP1.
Yingchao HanZhihua OuyangRichard A WawroseStephen H ChenMaximiliane HallbaumQing DongEmily DandoYing TangBing WangJoon Y LeeJeremy D ShawJames D KangGwendolyn A SowaNam N VoPublished in: European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society (2021)
AAV-NFκB-hTIMP1 is a novel inducible transgene delivery system. NFκB regulatory elements ensure that hTIMP1 expression occurs only with inflammation, which is central to IDD development. Unlike previous inducible systems, the AAV-NFκB-hTIMP1 construct is dependent on endogenous factors, which minimizes potential side effects caused by constitutive transgene overexpression. It also prevents the unnecessary production of transgene products in cells that do not require therapy.
Keyphrases
- signaling pathway
- oxidative stress
- lps induced
- poor prognosis
- induced apoptosis
- pi k akt
- nuclear factor
- cell cycle arrest
- transcription factor
- cell proliferation
- inflammatory response
- public health
- gene therapy
- binding protein
- long non coding rna
- stem cells
- risk assessment
- mouse model
- cell death
- endoplasmic reticulum stress
- immune response
- climate change
- smoking cessation