Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension.

Resveratrol, a secondary plant metabolite, and its derivatives, including piceid, show several potential health-related biological activities. However, resveratrol production is uncommon in plants; thus, resveratrol-enriched rice (DJ526) is produced for its nutritional and therapeutic value. Here, a DJ526 cell suspension was treated with various elicitors to determine its resveratrol-production potential and elicit its biological activity. Treatments with most elicitors produced more piceid than resveratrol; as elicitation periods increased, the average piceid levels were 75-fold higher than resveratrol levels. This increase is associated with glycosylation during growth and development. The duration of exposure and concentrations of elicitors were crucial factors affecting resveratrol synthase expression. Of all the elicitors tested, jasmonic acid and methyl jasmonate (MeJA) were strong elicitors; they increased resveratrol production to ≤115.1 μg g-1 (total resveratrol and piceid content). Moreover, 5 μM of MeJA increased total resveratrol production by >96.4% relative to the control production. In addition, the extract of cell suspension treated with 5 μM of MeJA significantly reduced melanin content and cellular tyrosinase activity (24.2% and 21.5% relative to the control, respectively) in melan-a cells without disturbing cell viability. Overall, elicitation can enhance resveratrol production and elicit the biological activity of the compound, in this case, its anti-melanogenic activities, in DJ526 cell suspension.