A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.

Wei ShengSetare Tahmasebi NickElizabeth M SantosXinliang DingJun ZhangChrysoula VasileiouJames H GeigerBabak Borhan

Published in: Angewandte Chemie (International ed. in English) (2018)

FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.

Keyphrases

high resolution
protein protein
amino acid
randomized controlled trial
binding protein
cystic fibrosis
photodynamic therapy
fluorescence imaging
fluorescent probe
single molecule
quantum dots
drug release
energy transfer