Pubescenosides E⁻K, Seven New Triterpenoid Saponins from the Roots of Ilex pubescens and Their Anti-Inflammatory Activity.
Xiaoxu QiaoMengying JiYunda YaoLeilei MaJinjun WuGuochao LiaoHua ZhouZhongqiu LiuPeng WuPublished in: Molecules (Basel, Switzerland) (2018)
Seven new triterpenoid saponins (1⁻7), together with three known ones (8⁻10), were isolated from Ilex pubescens. Elucidation of their structures was performed based on high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS), infrared spectra (IR), and nuclear magnetic resonance (NMR) spectroscopic data. The anti-inflammatory activity of the isolates toward lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages was investigated. The results demonstrated that compounds 3, 5, and 6 inhibited the expression of inducible nitric oxide synthase (iNOS) protein in comparison with LPS stimulation in RAW264.7 cells.
Keyphrases
- mass spectrometry
- high resolution
- nitric oxide synthase
- magnetic resonance
- inflammatory response
- nitric oxide
- liquid chromatography
- ms ms
- induced apoptosis
- gas chromatography
- high performance liquid chromatography
- anti inflammatory
- poor prognosis
- capillary electrophoresis
- toll like receptor
- cell cycle arrest
- binding protein
- tandem mass spectrometry
- molecular docking
- lps induced
- high speed
- electronic health record
- contrast enhanced
- multiple sclerosis
- atomic force microscopy
- immune response
- oxidative stress
- magnetic resonance imaging
- signaling pathway
- data analysis
- molecular dynamics simulations
- small molecule
- long non coding rna
- deep learning
- molecular dynamics
- solid phase extraction
- solid state