Recording morphogen signals reveals origins of gastruloid symmetry breaking.
Harold M McNamaraSabrina C SolleyBritt AdamsonMichelle M ChanJared E ToettcherPublished in: bioRxiv : the preprint server for biology (2023)
Symmetry-breaking gastruloid protocol where Wnt signaling evolves from a uniform high state to a single posterior domain.Synthetic gene circuits record Wnt, Nodal and BMP signaling with high time resolution.Heterogeneity in Wnt signaling at 96 h predicts cells' future positions and types.Wnt activity differences are influenced by earlier heterogeneity in Nodal/BMP activity.
Keyphrases
- mesenchymal stem cells
- induced apoptosis
- stem cells
- single cell
- cell proliferation
- neoadjuvant chemotherapy
- lymph node
- randomized controlled trial
- cell cycle arrest
- genome wide
- copy number
- transcription factor
- gene expression
- signaling pathway
- endoplasmic reticulum stress
- squamous cell carcinoma
- rectal cancer
- locally advanced