InterMEL: An international biorepository and clinical database to uncover predictors of survival in early-stage melanoma.
Irene OrlowKeimya D SadeghiSharon N EdmistonJessica M KenneyCecilia LezcanoJames S WilmottAnne E CustRichard A ScolyerGraham J MannTim K LeeHazel BurkeValerie JakrotPing ShangPeter M FergusonTawny W BoyceJennifer S KoPeter NgoPauline FunchainJudy R ReesKelli O'ConnellHonglin HaoEloise ParrishKathleen ConwayPaul B GoogeDavid W OllilaStergios J MoschosEva HernandoDouglas HannifordDiana ArgibayChristopher I AmosJeffrey E LeeIman OsmanLi LuoPei Fen KuanArshi AuroraBonnie E Gould RothbergMarcus W BosenbergMeg R GerstenblithCheryl ThompsonPaul N BognerIvan P GorlovSheri L HolmenElise K BrunsgaardYvonne M SaengerRonglai ShenVenkatraman SeshanEduardo NagoreMarc S ErnstoffKlaus J BusamColin B BeggNancy E ThomasMarianne Berwicknull nullPublished in: PloS one (2023)
Our experience with many archival tissues demonstrates that with careful management of tissue processing and quality control it is possible to conduct multi-omic studies in a complex multi-institutional setting for investigations involving minute quantities of FFPE tumors, as in studies of early-stage melanoma. The study describes, for the first time, the optimal strategy for obtaining archival and limited tumor tissue, the characteristics of the nucleic acids co-extracted from a unique cell lysate, and success rate in downstream applications. In addition, our findings provide an estimate of the anticipated attrition that will guide other large multicenter research and consortia.