The pluripotency transcription factor OCT4 represses heme oxygenase-1 gene expression.
María Victoria PetroneAyelén ToroCamila Vazquez EchegarayMarcos Gabriel FranciaClaudia SolariMaria Soledad CosentinoElba VazquezAlejandra S GubermanPublished in: FEBS letters (2021)
In embryonic stem (ES) cells, oxidative stress control is crucial for genomic stability, self-renewal, and cell differentiation. Heme oxygenase-1 (HO-1) is a key player of the antioxidant system and is also involved in stem cell differentiation and pluripotency acquisition. We found that the HO-1 gene is expressed in ES cells and induced after promoting differentiation. Moreover, downregulation of the pluripotency transcription factor (TF) OCT4 increased HO-1 mRNA levels in ES cells, and analysis of ChIP-seq public data revealed that this TF binds to the HO-1 gene locus in pluripotent cells. Finally, ectopic expression of OCT4 in heterologous systems repressed a reporter carrying the HO-1 gene promoter and the endogenous gene. Hence, this work highlights the connection between pluripotency and redox homeostasis.
Keyphrases
- induced apoptosis
- transcription factor
- oxidative stress
- gene expression
- cell cycle arrest
- genome wide
- copy number
- pi k akt
- signaling pathway
- dna methylation
- healthcare
- genome wide identification
- emergency department
- poor prognosis
- cell proliferation
- single cell
- mental health
- ischemia reperfusion injury
- dna damage
- embryonic stem cells
- artificial intelligence
- data analysis
- dna binding
- drug induced
- genome wide association study
- heat shock