Therapeutic efficacy of rolipram delivered by PgP nanocarrier on secondary injury and motor function in a rat TBI model.
Christian MacksDaun JeongJeoung Soo LeePublished in: Nanomedicine (London, England) (2022)
Aim: To develop poly(lactide- co -glycolide)-graft-polyethylenimine (PgP) as a nanocarrier for the delivery of rolipram (Rm) and evaluate the therapeutic efficacy of Rm-loaded PgP (Rm-PgP) on secondary injury and motor function in a rat traumatic brain injury (TBI) model. Materials & methods: Rm-PgP was injected in the injured brain lesion immediately after TBI using a microinjection pump. Secondary injury pathologies such as inflammatory response, apoptosis and astrogliosis were assessed by histological analysis and functional recovery was assessed by assorted motor function tests. Results: Rm-PgP restored cyclic adenosine monophosphate level in the injured brain close to the sham level and Rm-PgP treatment reduced lesion volume, neuroinflammation and apoptosis and improved motor function at 7 days post-TBI. Conclusion: One single injection of Rm-PgP can be effective for acute mild TBI treatment.
Keyphrases
- traumatic brain injury
- oxidative stress
- severe traumatic brain injury
- inflammatory response
- drug delivery
- endoplasmic reticulum stress
- cell death
- mild traumatic brain injury
- white matter
- cell cycle arrest
- cerebral ischemia
- liver failure
- cancer therapy
- acute respiratory distress syndrome
- signaling pathway
- ultrasound guided
- pi k akt