A basic ddRADseq two-enzyme protocol performs well with herbarium and silica-dried tissues across four genera.

Ingrid E Jordon-Thaden James B BeckCatherine A RushworthMichael D WindhamNicolas DiazJason T CantleyChristopher T MartineCarl J Rothfels

Published in: Applications in plant sciences (2020)

These results suggest that herbarium samples can be incorporated into ddRADseq project designs, and that specimen age can be used as a rapid on-site guide for sample choice. The detailed protocol we provide will allow users to pursue herbarium-based ddRADseq projects that minimize the expenses associated with fieldwork and sample evaluation.

Keyphrases

quality improvement
randomized controlled trial
gene expression
loop mediated isothermal amplification
decision making
quantum dots
clinical evaluation