Expression of HLA-DQA1 and HLA-DQB1 genes in B lymphocytes, monocytes and whole blood.
M ZajacovaA Kotrbova-KozakMarie CernaPublished in: International journal of immunogenetics (2018)
Differential expression of HLA-DQA1 and HLA-DQB1 gene alleles was analysed in three different cell populations isolated from peripheral blood-B lymphocytes, monocytes and whole-blood cells. Interallelic differences in mRNA levels were observed: DQA1*03 alleles were among the most expressed in all cell types, whereas DQA1*05 alleles were least expressed in whole blood and monocytes and among the most expressed in B cells. For DQB1 gene, DQB1*06 group of alleles were the most expressed, and DQB1*02 group the least expressed within all cell populations examined. In comparison with the rest alleles, DQB1*06 and DQB1*05:02 alleles have higher expression in monocytes than in B cells, professional antigen-presenting cells. Cell type-specific regulation of expression was observed as well, with higher and more balanced expression of alleles in B lymphocytes compared to monocytes.
Keyphrases
- peripheral blood
- poor prognosis
- single cell
- induced apoptosis
- dendritic cells
- binding protein
- genome wide
- cell therapy
- cell cycle arrest
- genome wide identification
- long non coding rna
- oxidative stress
- dna methylation
- endoplasmic reticulum stress
- signaling pathway
- case report
- mesenchymal stem cells
- genome wide analysis
- bioinformatics analysis