A Method for the Elution of Anti-CD20 With an EDTA/Glycine Acid Solution for Accurate Immunophenotyping of B Lymphocytes Sensitized With Rituximab.

Eli Fuentes-ChávezNereida Méndez-RamírezMarvin M MaltezRosario Salazar-RiojasDavid Gómez-AlmaguerJosé Carlos Jaime-Pérez

Published in: American journal of clinical pathology (2021)

Rituximab interfered with the flow cytometry protocol for CD20 determination on normal and neoplastic B cells. The EGA method efficiently eluted rituximab, allowing for accurate identification of CD20-positive B cells.

Keyphrases

flow cytometry
diffuse large b cell lymphoma
chronic lymphocytic leukemia
hodgkin lymphoma
nk cells
high resolution
randomized controlled trial