Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress.
Weijian BeiYujiao WangJianmei ChenJingjing ZhangLexun WangZhanhui GuYinming HuYijian HuangWei XuZili LeiJinyan CaiJiao GuoPublished in: Evidence-based complementary and alternative medicine : eCAM (2019)
FTZ treatment significantly improved the alteration in the level of SOD, CAT, Bcl-2, caspase-3, and MDA coupled with β cell dysfunction in diabetic rats. Oxidative stress in INS-1 cells was closely associated with a higher rate of apoptosis, increased production of ROS and MDA, enhanced Bax expression, and caspase-3, -9 activities and markedly decreased protein expression of Mn-SOD and CAT. FTZ-containing serum incubation notably reversed the high-glucose-evoked increase in cell apoptosis, production of ROS and MDA, and Bax protein levels. Furthermore, FTZ stimulation upregulated the expression levels of several genes, including Mn-SOD, CAT, and Bcl-2/Bcl-xl. In addition, FTZ decreased the intracellular activity of caspase-3, -9 in INS-1 cells. FTZ protected β-cells from oxidative stress induced by high glucose in vivo and in vitro. The beneficial effect of FTZ was closely associated with a decrease in the activity of caspase-3, -9 and intracellular production of ROS, MDA, and Bax coupled with an increase in the expression of Mn-SOD, CAT, and Bcl-2/Bcl-xl.
Keyphrases
- high glucose
- induced apoptosis
- oxidative stress
- diabetic rats
- cell cycle arrest
- cell death
- endothelial cells
- dna damage
- endoplasmic reticulum stress
- poor prognosis
- breast cancer cells
- reactive oxygen species
- ischemia reperfusion injury
- amyotrophic lateral sclerosis
- signaling pathway
- binding protein
- single cell
- cell therapy
- pi k akt
- long non coding rna
- cell proliferation
- room temperature
- transcription factor
- gene expression
- metal organic framework
- bone marrow
- protein protein