Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.

Barbara Pfister Jessica M Shields Tobias Kockmann Jonas Grossmann Melanie R Abt Martha Stadler Samuel C Zeeman

Published in: BMC biology (2022)

Our results provide a quantitative framework for the in silico design of improved starch biosynthetic processes in plants. Our study also exemplifies a workflow for the rational tuning of a complex pathway in yeast, starting from the selection and evaluation of expression modules to multi-gene assembly and targeted protein monitoring during the biosynthetic process.

Keyphrases

cell wall
saccharomyces cerevisiae
poor prognosis
binding protein
copy number
molecular docking
genome wide
high resolution
cancer therapy
protein protein
small molecule
gene expression
long non coding rna
mass spectrometry
molecular dynamics simulations