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Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource.

Martin KapunJoaquin C B NunezMaría Bogaerts-MárquezJesús Murga-MorenoMargot ParisJoseph OuttenMarta Coronado-ZamoraCourtney TernOmar Rota-StabelliMaria P García GuerreiroSònia CasillasDorcas J OrengoEva PuermaMaaria KankareLino OmettoVolker LoeschckeBanu Şebnem ÖnderJessica K AbbottStephen W SchaefferSubhash RajpurohitEmily L BehrmanMads Fristrup SchouThomas J S MerrittBrian P LazzaroAmanda Glaser-SchmittEliza ArgyridouFabian StaubachYun WangEran TauberSvitlana V SergaDaniel K FabianKelly A DyerChristopher W WheatJohn ParschAnnabella KönigerMarija Savic VeselinovicMarina Stamenkovic-RadakMihailo JelicAntonio J Buendía-RuízMaria Josefa Gómez-JuliánMaria Luisa Espinosa-JimenezFrancisco D Gallardo-JiménezAleksandra PatenkovićKatarina EricMarija TanaskovicAnna UllastresLain GuioMiriam MerencianoSara Guirao-RicoVivien HorváthDarren J ObbardElena PasyukovaVladimir E AlatortsevCristina P VieiraJorge VieiraJorge Roberto TorresIryna KozeretskaOleksandr M MaistrenkoCatherine Montchamp-MoreauDmitry V MukhaHeather E MachadoKeric LambTânia PauloLeeban YusufAntonio BarbadillaDmitri PetrovPaul SchmidtJosefa GonzalezThomas FlattAlan O Bergland
Published in: Molecular biology and evolution (2022)
Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome data sets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate data sets, often generated using different sequencing technologies and bioinformatic pipelines, which hampers our ability to address questions about the evolution of this species. Here we address these issues by developing a bioinformatics pipeline that maps pooled sequencing (Pool-Seq) reads from D. melanogaster to a hologenome consisting of fly and symbiont genomes and estimates allele frequencies using either a heuristic (PoolSNP) or a probabilistic variant caller (SNAPE-pooled). We use this pipeline to generate the largest data repository of genomic data available for D. melanogaster to date, encompassing 271 previously published and unpublished population samples from over 100 locations in >20 countries on four continents. Several of these locations have been sampled at different seasons across multiple years. This data set, which we call Drosophila Evolution over Space and Time (DEST), is coupled with sampling and environmental metadata. A web-based genome browser and web portal provide easy access to the SNP data set. We further provide guidelines on how to use Pool-Seq data for model-based demographic inference. Our aim is to provide this scalable platform as a community resource which can be easily extended via future efforts for an even more extensive cosmopolitan data set. Our resource will enable population geneticists to analyze spatiotemporal genetic patterns and evolutionary dynamics of D. melanogaster populations in unprecedented detail.
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