Ischemic Stroke Induces Skeletal Muscle Damage and Alters Transcriptome Profile in Rats.
Hu QiXiangyu LiXiumeng ZhangBin LiDan TianDejian WangRuocong YangNan ZengPublished in: Journal of clinical medicine (2023)
To establish pathological features of skeletal muscle post-stroke and to provide a background for promising interventions. Adult male SD rats were selected and randomly divided into a control group, a sham group, and a middle cerebral artery occlusion (MCAO) group. The tolerance and capability of exercise were separately collected on days 1, 3, 5, and 7 after the MCAO operation. The neurological deficits, brain infarct volume, soleus histopathology, mRNA-seq analysis, flow cytometry, immunofluorescence, and protein expression analysis were performed on the seventh day. Rats in the MCAO group showed that soleus tissue weight, pulling force, exercise capacity, endurance, and muscle structure were significantly decreased. Moreover, the RNA sequencing array revealed that mitochondrial-mediated autophagy was the critical pathological process, and the result of transcriptomic findings was confirmed at the translational level. The mitochondrial membrane potential and the mfn2 and p62 protein expression were decreased, and the Beclin-1, ATG5, Parkin, PINK1, LC3B, and Drp1 expression were upregulated; these results were consistent with immunohistochemistry. This is the first report on the pathological features of limbic symptoms on day 7 after MCAO surgery in rats. In addition, we further confirmed that autophagy is one of the main causative mechanisms of reduced muscle function after stroke.
Keyphrases
- skeletal muscle
- single cell
- oxidative stress
- middle cerebral artery
- physical activity
- rna seq
- high intensity
- flow cytometry
- insulin resistance
- cell death
- genome wide
- signaling pathway
- poor prognosis
- resistance training
- endoplasmic reticulum stress
- binding protein
- high throughput
- acute myocardial infarction
- coronary artery disease
- white matter
- clinical trial
- high resolution
- body mass index
- metabolic syndrome
- adipose tissue
- weight loss
- depressive symptoms
- single molecule
- blood brain barrier
- multiple sclerosis
- internal carotid artery
- small molecule