A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer.

Kazuo ImaiNorihito TarumotoKazuhisa MisawaLucky Ronald RuntuweneJun SakaiKyoko HayashidaYuki EshitaRyuichiro MaedaJosef TudaTakashi MurakamiShigefumi MaesakiYutaka SuzukiJunya YamagishiTakuya Maeda

Published in: BMC infectious diseases (2017)

Our diagnostic method combined with LAMP and MinION™ could become a simple and accurate tool for the identification of human Plasmodium species, even in resource-limited situations.

Keyphrases

loop mediated isothermal amplification
sensitive detection
plasmodium falciparum
endothelial cells
high resolution
single molecule
pluripotent stem cells
mass spectrometry
quantum dots
solid state