Qualitative and Quantitative Analysis of the Major Constituents in WLJ Herbal Tea Using Multiple Chromatographic Techniques.
Chao-Zhan LinRun-Jing ZhangYu-Feng YaoXiao-Dan HuangRong-Bo ZhengBo-Jian WuChen-Chen ZhuPublished in: Molecules (Basel, Switzerland) (2018)
Quality control of Chinese herbal tea remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling and famous brand of beverage in China, Wanglaoji Herbal Tea (WLJHT), via a full component quantitative analysis. In this paper, a total of thirty-two representative constituents were identified or tentatively characterized using ultra-high performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Moreover, the quantitative analyses of fourteen constituents were performed by high performance liquid chromatography with a triple quadruple tandem mass spectrometry (HPLC-MS/MS) method and saccharide compositions of WLJHT were also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on a Hilic column, separately. Using multiple chromatographic techniques presented a good precision, sensitivity, repeatability and stability, and was successfully applied to analyze 16 batches of WLJHT samples. Therefore, it would be a reliable and useful approach for the quality control of WLJHT.
Keyphrases
- quality control
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- gas chromatography
- mass spectrometry
- high resolution
- high resolution mass spectrometry
- ms ms
- essential oil
- healthcare
- helicobacter pylori
- systematic review
- helicobacter pylori infection
- cross sectional
- monte carlo
- computed tomography