<div>Human eccrine sweat contains numerous biomarkers which can provide information on health,</div><div>performance, and aging. Non-invasive collection and measurement of biomarkers has become</div><div>especially important in recent times given viral outbreaks like SARS-CoV-2. In the current study</div><div>we describe a method of sweat collection from palmar surfaces in participants via surface capture</div><div>using glass beads and the resulting analysis of biomarkers from very low volumes of sweat using</div><div>liquid chromatography mass spectrometry with selected ion monitoring. Study participants</div><div>underwent a cognitive and physical stress task with easy and hard conditions with sweat being</div><div>collected after each task. Resulting analysis found a signal for 22 steroid biomarkers and we</div><div>report detailed information on selected biomarkers, given their applicability to timely real-world</div><div>exemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,</div><div>and 20a/b-dihydrocortisone.</div><div><br></div>.