Transcriptional Reprogramming of Autographa Californica Multiple Nucleopolyhedrovirus Chitinase and Cathepsin Genes Enhances Virulence.
Jeffrey J HodgsonA Lorena PassarelliPeter J KrellPublished in: Viruses (2023)
The baculoviral chitinase (CHIA) and cathepsin (V-CATH) enzymes promote terminal insect host liquefaction, which aids viral progeny dissemination. Recombinant Autographa californica nucleopolyhedrovirus (AcMNPV)-derived viruses were previously generated with reprogrammed chiA transcription by replacing the native promoter with the AcMNPV polyhedrin ( polh ) or core protein ( p6.9 ) promoter sequences, but of both these chiA -reprogrammed viruses lacked v-cath transcription and V-CATH enzymatic activity. Here, we report that dual p6.9 / polh promoter reprogramming of the adjacent chiA/v-cath genes resulted in modulated temporal transcription of both genes without impacting infectious budded virus production. These promoter changes increased CHIA and V-CATH enzyme activities in infected Spodoptera frugiperda -derived cultured cells and Trichoplusia ni larvae. In addition, larvae infected with the dual reprogrammed virus had earlier mortalities and liquefaction. This recombinant baculovirus, lacking exogenous genomic elements and increased chiA/v-cath expression levels, may be desirable for and amenable to producing enhanced baculovirus-based biopesticides.
Keyphrases
- transcription factor
- genome wide identification
- dna methylation
- genome wide
- gene expression
- aedes aegypti
- induced apoptosis
- poor prognosis
- bioinformatics analysis
- escherichia coli
- sars cov
- binding protein
- cell free
- risk assessment
- long non coding rna
- genetic diversity
- antiretroviral therapy
- amino acid
- heat shock
- signaling pathway
- heavy metals
- metal organic framework