Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals.
Parul SinghSyed Azmal AliPublished in: Veterinary sciences (2021)
Humans are sorely over-dependent on livestock for their daily basic need of food in the form of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the opportunity for more significant gains and production in a short span of time. One of the best strategies is the genetic alteration of livestock to enhance the efficiency of food production (e.g., meat and milk), animal health, and welfare (animal population and disease). Moreover, genome engineering in the bovine is majorly focused on subjects such as disease resistance (e.g., tuberculosis), eradicate allergens (e.g., beta-lactoglobulin knock-out), products generation (e.g., meat from male and milk from female), male or female birth specifically (animal sexing), the introduction of valuable traits (e.g., stress tolerance and disease resistance) and their wellbeing (e.g., hornlessness). This review addressed the impressive genome engineering method CRISPR, its fundamental principle for generating highly efficient target-specific guide RNA, and the accompanying web-based tools. However, we have covered the remarkable roadmap of the CRISPR method from its conception to its use in cattle. Additionally, we have updated the comprehensive information on CRISPR-based gene editing in cattle.
Keyphrases
- genome wide
- crispr cas
- genome editing
- dna methylation
- highly efficient
- copy number
- healthcare
- public health
- mycobacterium tuberculosis
- health information
- physical activity
- pulmonary tuberculosis
- pregnant women
- hiv aids
- antiretroviral therapy
- hepatitis c virus
- climate change
- drug induced
- hiv infected
- gestational age
- adverse drug