Secondary modification of oxidatively-modified proline N-termini for the construction of complex bioconjugates.
Johnathan C MazaAlexandra V RamseyMeire MehareShane W KrskaCraig A ParishMatthew B FrancisPublished in: Organic & biomolecular chemistry (2020)
A convenient two-step method is reported for the ligation of alkoxyamine- or hydrazine-bearing cargo to proline N-termini. Using this approach, bifunctional proline N-terminal bioconjugates are constructed and proline N-terminal proteins are immobilized.