Elicitation of Inhibitory Effects for AGE-Induced Oxidative Stress in Rotator Cuff-Derived Cells by Apocynin.
Takahiro FurukawaTakashi KurosawaYutaka MifuneAtsuyuki InuiHanako NishimotoYasuhiro UedaTakeshi KataokaKohei YamauraShintaro MukoharaTomoya YoshikawaIssei ShinoharaTatsuo KatoShuya TanakaMasaya KusunoseYuichi HoshinoTakehiko MatsushitaRyosuke KurodaPublished in: Current issues in molecular biology (2023)
Advanced glycation end-products (AGEs) play a critical supportive role during musculoskeletal disorders via glycosylation and oxidative stress. Though apocynin, identified as a potent and selective inhibitor of NADPH oxidase, has been reported to be involved in pathogen-induced reactive oxygen species (ROS), its role in age-related rotator cuff degeneration has not been well clarified. Therefore, this study aims to evaluate the in vitro effects of apocynin on human rotator cuff-derived cells. Twelve patients with rotator cuff tears (RCTs) participated in the study. Supraspinatus tendons from patients with RCTs were collected and cultured. After the preparation of RC-derived cells, they were divided into four groups (control group, control + apocynin group, AGEs group, AGEs + apocynin group), and gene marker expression, cell viability, and intracellular ROS production were evaluated. The gene expression of NOX, IL-6, and the receptor for AGEs (RAGE) was significantly decreased by apocynin. We also examined the effect of apocynin in vitro. The results showed that ROS induction and increasing apoptotic cells after treatment of AGEs were significantly decreased, and cell viability increased considerably. These results suggest that apocynin can effectively reduce AGE-induced oxidative stress by inhibiting NOX activation. Thus, apocynin is a potential prodrug in preventing degenerative changes of the rotor cuff.
Keyphrases
- rotator cuff
- reactive oxygen species
- induced apoptosis
- cell cycle arrest
- cell death
- gene expression
- oxidative stress
- dna damage
- endoplasmic reticulum stress
- endothelial cells
- signaling pathway
- hydrogen peroxide
- dna methylation
- diabetic rats
- poor prognosis
- heat shock
- pi k akt
- cancer therapy
- mass spectrometry
- heat stress
- genome wide
- climate change
- copy number