Functional analysis of Salmonella Typhi adaptation to survival in water.
Robert A KingsleyGemma LangridgeSarah E SmithCarine MakendiMaria FookesTom M WilemanMoataz Abd El GhanyA Keith TurnerZoe A DysonSushmita SridharDerek PickardSally KayNicholas FeaseyVanessa WongLars BarquistGordon DouganPublished in: Environmental microbiology (2019)
Contaminated water is a major risk factor associated with the transmission of Salmonella enterica serovar Typhi (S. Typhi), the aetiological agent of human typhoid. However, little is known about how this pathogen adapts to living in the aqueous environment. We used transcriptome analysis (RNA-seq) and transposon mutagenesis (TraDIS) to characterize these adaptive changes and identify multiple genes that contribute to survival. Over half of the genes in the S. Typhi genome altered expression level within the first 24 h following transfer from broth culture to water, although relatively few did so in the first 30 min. Genes linked to central metabolism, stress associated with arrested proton motive force and respiratory chain factors changed expression levels. Additionally, motility and chemotaxis genes increased expression, consistent with a scavenging lifestyle. The viaB-associated gene tviC encoding a glcNAc epimerase that is required for Vi polysaccharide biosynthesis was, along with several other genes, shown to contribute to survival in water. Thus, we define regulatory adaptation operating in S. Typhi that facilitates survival in water.
Keyphrases
- genome wide
- genome wide identification
- poor prognosis
- rna seq
- bioinformatics analysis
- genome wide analysis
- free survival
- dna methylation
- single cell
- transcription factor
- endothelial cells
- metabolic syndrome
- escherichia coli
- risk factors
- cardiovascular disease
- heavy metals
- crispr cas
- binding protein
- type diabetes
- weight loss
- cystic fibrosis
- drinking water
- risk assessment
- biofilm formation
- electron transfer