Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study.
Riccardo ZecchiPietro FranceschiLaura TigliDavide AmidaniChiara CatozziFrancesca RicciFabrizio SalomoneGiuseppe PieracciniBarbara PioselliValentina MileoPublished in: Analytical and bioanalytical chemistry (2021)
Corticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard's reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution
- chronic obstructive pulmonary disease
- allergic rhinitis
- gas chromatography
- pulmonary hypertension
- high performance liquid chromatography
- randomized controlled trial
- capillary electrophoresis
- lung function
- high resolution mass spectrometry
- emergency department
- cystic fibrosis
- molecularly imprinted
- gas chromatography mass spectrometry
- electronic health record
- loop mediated isothermal amplification
- respiratory tract