Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium.
Marek KieliszekKatarzyna BierlaJavier Jiménez-LamanaAnna Maria KotJaime Alcántara-DuránKamil PiwowarekStanisław BłażejakRyszard ŁobińskiPublished in: International journal of molecular sciences (2020)
Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20-30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.
Keyphrases
- ms ms
- saccharomyces cerevisiae
- induced apoptosis
- high resolution
- cell wall
- body mass index
- multiple sclerosis
- stem cells
- staphylococcus aureus
- single cell
- oxidative stress
- cystic fibrosis
- cell therapy
- bone marrow
- stress induced
- escherichia coli
- simultaneous determination
- nitric oxide
- mesenchymal stem cells
- hydrogen peroxide
- endoplasmic reticulum stress
- weight loss
- tandem mass spectrometry
- high performance liquid chromatography
- heat stress