Visible Light-Guided Gene Delivery with Nonviral Supramolecular Block Copolymer Vectors.
Wumaier YasenBei LiAliya AiniZiying LiYue SuLinzhu ZhouDongbo GuoQiuhui QianDong ChenXinyuan ZhuRuijiao DongPublished in: ACS applied materials & interfaces (2023)
To achieve efficient gene delivery in vitro or in vivo , nonviral vectors should have excellent biostability across cellular and tissue barriers and also smart stimuli responsiveness toward controlled release of therapeutic genes into the cell nucleus. However, it remains a key challenge to effectively combine the biostability of covalent polymers with the stimuli responsiveness of noncovalent polymers into one nonviral vehicle. In this work, we report the construction of a kind of cationic supramolecular block copolymers (SBCs) through noncovalent polymerization of β-cyclodextrin/azobenzene-terminated pentaethylenehexamine (DMA-Azo-PEHA-β-CD) in aqueous media using β-CD-monosubstituted poly(ethylene glycol) (PEG-β-CD) as a supramolecular initiator. The resultant SBC exhibits superior biostability, biocompatibility, and light/pH dual-responsive characteristics, and it also demonstrates efficient plasmid DNA condensation capacity and the ability to rapidly release plasmid DNA into cells driven by visible light (450 nm). Eventually, this SBC-based delivery system demonstrates visible light-induced enhancement of gene delivery in both COS-7 and HeLa cells. We anticipate that this work provides a facile and robust strategy to enhance gene delivery in vitro or in vivo via visible light-guided manipulation of genes, further achieving safe, highly efficient, targeting gene therapy for cancer.
Keyphrases
- visible light
- highly efficient
- induced apoptosis
- cell cycle arrest
- genome wide
- escherichia coli
- circulating tumor
- genome wide identification
- cell free
- endoplasmic reticulum stress
- single cell
- cancer therapy
- drug delivery
- ionic liquid
- cell therapy
- nk cells
- photodynamic therapy
- dna methylation
- pi k akt
- young adults
- gene expression
- oxidative stress
- bioinformatics analysis
- copy number
- mass spectrometry
- papillary thyroid
- squamous cell
- gold nanoparticles
- cell proliferation
- mesenchymal stem cells
- transcription factor
- light emitting