Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.

The microbial production of important industrial enzymes in recombinant systems can be improved by applying newly available molecular tools. Overall, the work presented here on the optimisation of phytase production in P. pastoris contributes to the improved understanding of recombinant protein folding and secretion in this important yeast microbial production host.