High-throughput screening of clinically approved drugs that prime nonviral gene delivery to human Mesenchymal stem cells.
Tyler KozisekAndrew HamannAlbert NguyenMichael MillerSarah PlautzAngela K PannierPublished in: Journal of biological engineering (2020)
Our screen of the NCC is the first high-throughput and drug-repurposing approach to identify nonviral gene delivery priming compounds in two donors of hMSCs. Priming compounds and classes identified in this screen suggest that modulation of proliferation, mitochondrial function, and apoptosis is vital for enhancing nonviral gene delivery to hMSCs.
Keyphrases
- high throughput
- mesenchymal stem cells
- endothelial cells
- single cell
- oxidative stress
- endoplasmic reticulum stress
- signaling pathway
- umbilical cord
- cell death
- induced pluripotent stem cells
- cell cycle arrest
- drug induced
- bone marrow
- pluripotent stem cells
- cell therapy
- cell proliferation
- emergency department
- kidney transplantation
- drug administration