Rational engineering of industrial S. cerevisiae: towards xylitol production from sugarcane straw.
Fellipe da Silveira Bezerra de MelloCarla ManeiraFrank Uriel Lizarazo SuarezSheila NagamatsuBeatriz VargasCarla VieiraThais SecchesAlessando L V CoradiniMaria Augusta de Carvalho SilvelloRosana GoldbeckGonçalo Amarante Guimaraes PereiraGleidson Silva TeixeiraPublished in: Journal, genetic engineering & biotechnology (2022)
Although widely used in several S. cerevisiae strains, this is the first report of CRISPR-Cas9 editing major yeast of the Brazilian bioethanol industry. Fermentative assays of xylose consumption revealed that NADPH availability is closely related to mutant strains' performance. We also pioneer the use of sugarcane straw as a substrate for xylitol production. Finally, we demonstrate how industrial background SA-1 is a compelling chassis for the second-generation industry, given its inhibitor tolerance and better redox environment that may favor production of reduced sugars.