Toxicological Evaluation of Luminescent Silica Nanoparticles as New Drug Nanocarriers in Different Cancer Cell Lines.
Gonçalo A MarceloJessica Ariana-MachadoMaria EneaHelena CarmoBenito Rodríguez-GonzálezJosé Luis CapeloCarlos LodeiroElisabete OliveiraPublished in: Materials (Basel, Switzerland) (2018)
Luminescent mesoporous silica nanoparticles, CdTeQDs@MNs@PEG1, SiQDs@Isoc@MNs and SiQDs@Isoc@MNs@PEG2, were successfully synthetized and characterized by SEM, TEM, XRD, N₂ nitrogen isotherms, ¹H NMR, IR, absorption, and emission spectroscopy. Cytotoxic evaluation of these nanoparticles was performed in relevant in vitro cell models, such as human hepatoma HepG2, human brain endothelial (hCMEC/D3), and human epithelial colorectal adenocarcinoma (Caco-2) cell lines. None of the tested nanoparticles showed significant cytotoxicity in any of the three performed assays (MTT/NR/ LDH) compared with the respective solvent and/or coating controls, excepting for CdTeQDs@MNs@PEG1 nanoparticles, where significant toxicity was noticed in hCMEC/D3 cells. The results presented reveal that SiQDs-based mesoporous silica nanoparticles are promising nanoplatforms for cancer treatment, with a pH-responsive drug release profile and the ability to load 80% of doxorubicin.
Keyphrases
- drug delivery
- drug release
- endothelial cells
- single cell
- high resolution
- quantum dots
- induced apoptosis
- squamous cell carcinoma
- emergency department
- sensitive detection
- walled carbon nanotubes
- high throughput
- oxidative stress
- gene expression
- pluripotent stem cells
- dna methylation
- solid state
- rectal cancer
- electronic health record
- adverse drug
- light emitting