Characterization of a Bacteriophage GEC_vB_Bfr_UZM3 Active against Bacteroides fragilis .
Nata BakuradzeMaia MerabishviliIa KusradzePieter-Jan CeyssensJolien OnseaWillem-Jan MetsemakersNino GrdzelishviliGuliko NatroshviliTamar TatrishviliDavit LazvliashviliNunu MitskevichJean-Paul PirnayNina ChanishviliPublished in: Viruses (2023)
Bacteroides fragilis is a commensal gut bacterium that is associated with a number of blood and tissue infections. It has not yet been recognized as one of the drug-resistant human pathogens, but cases of the refractory infections, caused by strains that are not susceptible to the common antibiotic regimes established for B. fragilis, have been more frequently reported. Bacteriophages (phages) were found to be a successful antibacterial alternative to antibiotic therapy in many cases of multidrug-resistant (MDR) bacterial infections. We have characterized the bacteriophage GEC_vB_Bfr_UZM3 (UZM3), which was used for the treatment of a patient with a chronic osteomyelitis caused by a B. fragilis mixed infection. Studied biological and morphological properties of UZM3 showed that it seems to represent a strictly lytic phage belonging to a siphovirus morphotype. It is characterized by high stability at body temperature and in pH environments for about 6 h. Whole genome sequencing analysis of the phage UZM3 showed that it does not harbor any known virulence genes and can be considered as a potential therapeutic phage to be used against B. fragilis infections.
Keyphrases
- multidrug resistant
- drug resistant
- pseudomonas aeruginosa
- gram negative
- acinetobacter baumannii
- escherichia coli
- endothelial cells
- stem cells
- klebsiella pneumoniae
- case report
- antimicrobial resistance
- genome wide
- cystic fibrosis
- mesenchymal stem cells
- gene expression
- drug induced
- transcription factor
- pluripotent stem cells
- silver nanoparticles