Let-7a-5p abrogates progression of papillary thyroid carcinoma cells by decreasing nuclear receptor subfamily 6 group a member 1-mediated lipogenesis.
Tao ZhaoJinghui SunXiangdong LuLingling LiuLin ChenWei ZhaoBin ZhouPublished in: Journal of biochemical and molecular toxicology (2023)
Increasing evidence shows that microRNAs (miRNAs) contribute vital roles in papillary thyroid carcinoma (PTC) carcinogenesis, proliferation, invasion, and so on. As the most common endocrine malignancy, there still have largely unknown molecular events. First, our analysis and open access database information indicates that the downregulation of let-7a-5p accelerates PTC progression. Next, lentivirus mediates the overexpression of let-7a-5p PTC cells, and found let-7a-5p suppressed cancer cells proliferation and invasion. Interestingly, bioinformatics analysis hints NR6A1 is the potential target gene of let-7a-5p. The regulation was validated by luciferase and quantitative reverse transcription polymerase chain reaction (qRT-PCR) in PTC tissue and the clinic tumors. Moreover, let-7a-5p regulated NR6A1 involved in PTC cells lipogensis in vitro and in vivo. Finally, let-7a-5p abrogates PCT xenograft tumors growth, NR6A1 expression and lipogenesis. Taken together, our data indicates that let-7a-5p suppresses PCT progression through decreased lipogenesis, the related let-7a-5p/NR6A1axis might be promising candidate targets for PTC treatment.
Keyphrases
- induced apoptosis
- signaling pathway
- lymph node metastasis
- cell cycle arrest
- papillary thyroid
- transcription factor
- cell proliferation
- poor prognosis
- bioinformatics analysis
- high fat diet induced
- lymph node
- endoplasmic reticulum stress
- primary care
- cell death
- healthcare
- emergency department
- type diabetes
- oxidative stress
- pi k akt
- machine learning
- electronic health record
- minimally invasive
- metabolic syndrome
- binding protein
- copy number
- insulin resistance
- dna methylation
- combination therapy
- replacement therapy
- climate change
- social media