Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice.
Wenhui XuJiayu GongYifei ChenYiru ChenShutong ChenYanyan WuYuan HeChenxu LiHaitao YuLin XiePublished in: Nutrients (2023)
This study aimed to explore the effects and mechanisms of maternal gestational diabetes mellitus (GDM) and selenium (Se) deficiency on the growth and glucose metabolism of offspring. Female C57BL/6J mice were divided into four groups as follows: a control group, a GDM group, a Se deficiency group, and a GDM with Se deficiency group. GDM animal models were established via S961. Pregnant mice fed their offspring until weaning. Then, offspring continued to be fed with a basic diet until adulthood. Body weight and fasting blood glucose were measured weekly. Se content, oxidative stress indicators, and the protein expression of the PI3K/Akt signaling pathway were detected. GDM increased susceptibility to obesity in lactating offspring, with gender differences observed in adult offspring. The effect of Se deficiency on SOD activity only appeared in female offspring during adulthood but was shown in male offspring during weaning though it disappeared during adulthood. GDM and Se deficiency increased the risk of abnormal glucose metabolism in female offspring from weaning to adulthood but gradually decreased in male offspring. The influence on the expression of PI3K/Akt signaling pathway-related proteins showed the same trend. GDM and Se deficiency affected the growth and glucose metabolism of offspring through oxidative stress and PI3K/Akt signaling pathway-related proteins, and gender differences existed.
Keyphrases
- pi k akt
- signaling pathway
- high fat diet
- blood glucose
- oxidative stress
- insulin resistance
- depressive symptoms
- cell proliferation
- induced apoptosis
- high fat diet induced
- replacement therapy
- epithelial mesenchymal transition
- blood pressure
- weight loss
- type diabetes
- adipose tissue
- intensive care unit
- dna damage
- poor prognosis
- early life
- metabolic syndrome
- glycemic control
- body mass index
- cell death
- amyotrophic lateral sclerosis
- binding protein
- extracorporeal membrane oxygenation