A novel imaging flow cytometry method for the detection of histone H4 acetylation in myeloid cells.

Suriyon UitrakulClaire HuttonGareth J Veal David Jamieson

Published in: European journal of clinical investigation (2019)

The percentage of acH4-positive myeloid cells has the potential to be used as a robust pharmacodynamic biomarker for the measurement of acH4 for HDACIs. The developed assay is now being utilised in a clinical trial involving the treatment of childhood ependymoma patients with valproic acid.

Keyphrases

induced apoptosis
flow cytometry
clinical trial
cell cycle arrest
bone marrow
dendritic cells
acute myeloid leukemia
endoplasmic reticulum stress
randomized controlled trial
immune response
study protocol
pi k akt
risk assessment
label free
phase iii
histone deacetylase