A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells.
Yu BaiEmmanuel CaussinusStefano LeoFritz BosshardtFaina MyachinaGregor RotMark D RobinsonChristian F LehnerPublished in: BMC genomics (2021)
Beyond a rich data resource for future analyses of transcriptional control within the readily tolerated range of an ectothermic animal, a novel reporter assay permitting quantitative characterization of CRE temperature dependence was developed. Our identification and functional dissection of the pst_E1 enhancer demonstrate the utility of resources and assay. The functional characterization of this CoolUp enhancer provides initial mechanistic insights into transcriptional upregulation induced by a shift to temperatures at the lower end of the readily tolerated range.
Keyphrases
- transcription factor
- induced apoptosis
- high throughput
- cell cycle arrest
- binding protein
- gene expression
- crispr cas
- signaling pathway
- cell proliferation
- endothelial cells
- endoplasmic reticulum stress
- poor prognosis
- high resolution
- current status
- electronic health record
- cell death
- oxidative stress
- long non coding rna
- deep learning
- mass spectrometry