Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis.
Ivan Sergeevich SazykinMaksim S MakarenkoLudmila Eugenevna KhmelevtsovaEkaterina SeliverstovaAlexander RakinMarina Alexandrovna SazykinaPublished in: MicrobiologyOpen (2019)
In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon-degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.
Keyphrases
- hydrogen peroxide
- oxidative stress
- gene expression
- poor prognosis
- induced apoptosis
- genome wide
- nitric oxide
- particulate matter
- dna methylation
- dna damage
- genome wide identification
- endoplasmic reticulum stress
- long non coding rna
- metal organic framework
- diabetic rats
- cell death
- binding protein
- signaling pathway
- genome wide analysis
- cell proliferation
- cell cycle arrest
- ischemia reperfusion injury
- air pollution
- heavy metals
- single molecule
- copy number
- risk assessment
- reactive oxygen species
- aqueous solution