Temporal Modulation of DNA Methylation and Gene Expression in Monolayer and 3D Spheroids of Dental Pulp Stem Cells during Osteogenic Differentiation: A Comparative Study.

Shalini RaikReetu ThakurVidya RattanNavin KumarArnab PalShalmoli Bhatta Charyya

Published in: Tissue engineering and regenerative medicine (2022)

The distinct cellular microenvironment induced changes in DNA methylation pattern and expression of epigenetic regulators such as DNMTs and TETs genes may lead to increase expression of osteogenic markers in 3D spheroid culture of DPSCs which make DPSCs spheroids suitable for osteogenic regeneration compared to monolayers.

Keyphrases

dna methylation
stem cells
gene expression
genome wide
mesenchymal stem cells
bone marrow
poor prognosis
copy number
cell therapy
binding protein
long non coding rna