miR-146a Protects against Staphylococcus aureus -Induced Osteomyelitis by Regulating Inflammation and Osteogenesis.
Chaolai JiangYiwei LinHaojie ShanWenyang XiaChenhao PanNan WangLihui ZhouYou-Shui GaoZubin ZhouXiaowei YuPublished in: ACS infectious diseases (2022)
Osteomyelitis is a Staphylococcus aureus -caused bone infection. In this study, the effects of miR-146a on osteomyelitis were evaluated. Using the osteoblast cell model and S. aureus -induced osteomyelitis mice model, we monitored the miR-146 expression and explored the effects of miR-146a on cell proliferation of osteoblasts, bone remodeling, osteoclastogenesis, inflammatory cytokine production, and bacterial burden. Upregulated miR-146a was found in mice with S. aureus -induced osteomyelitis. miR-146a attenuated S. aureus -induced cell loss of osteoblasts, rescued the expression of osteogenic markers, altered the bone remodeling, and inhibited inflammatory cytokine production and osteoclastogenesis. miR-146a knockout mice had higher S. aureus burden. In conclusion, miR-146a protects against S. aureus -induced osteomyelitis by regulating inflammation and osteogenesis.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- staphylococcus aureus
- high glucose
- poor prognosis
- diabetic rats
- oxidative stress
- cell cycle
- drug induced
- bone mineral density
- pi k akt
- mesenchymal stem cells
- bone marrow
- single cell
- type diabetes
- risk factors
- metabolic syndrome
- endothelial cells
- cystic fibrosis
- binding protein
- body composition
- cell therapy
- signaling pathway
- soft tissue
- inflammatory response
- skeletal muscle