Oblique-plane single-molecule localization microscopy for tissues and small intact animals.
Jeongmin KimMichal WojcikYuan WangSeonah MoonEmilia A ZinNadia MarnaniZachary L NewmanJohn G FlanneryKe XuXiang ZhangPublished in: Nature methods (2019)
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
Keyphrases
- single molecule
- drosophila melanogaster
- induced apoptosis
- living cells
- atomic force microscopy
- cell cycle arrest
- high resolution
- white matter
- resting state
- endoplasmic reticulum stress
- gene expression
- signaling pathway
- functional connectivity
- cell proliferation
- cerebral ischemia
- pi k akt
- diabetic retinopathy
- blood brain barrier
- subarachnoid hemorrhage
- zika virus
- single cell