Highly reliable creation of floxed alleles by electroporating single-cell embryos.

Monica F SentmanatJ Michael WhiteEvguenia KouranovaXiaoxia Cui

Published in: BMC biology (2022)

Floxed alleles can be reliably obtained in a predictable timeline using the improved method of electroporation of two gRNA/Cas9 ribonucleoprotein particles (RNPs) and two ssODNs.

Keyphrases

single cell
crispr cas
rna seq
genome editing
high throughput