Ascleposide, a natural cardenolide, induces anticancer signaling in human castration-resistant prostatic cancer through Na+ /K+ -ATPase internalization and tubulin acetylation.
Wohn-Jenn LeuChing-Ting WangJui-Ling HsuIh-Sheng ChenHsun-Shuo ChangJih-Hwa GuhPublished in: The Prostate (2020)
Ascleposide displays antiproliferative and apoptotic activities dependent on the inhibition of Na+ /K+ -ATPase pumping activity through p38 MAPK-mediated endocytosis of Na+ /K+ -ATPase and downregulation of α1 subunit, which in turn cause tubulin acetylation and cell cycle arrest. Cell apoptosis is ultimately triggered by the activation of caspase cascade attributed to mitochondrial damage through the downregulation of Bcl-2 and Mcl-1 protein expressions while upregulation of Bak protein levels. The data also suggest the potential of ascleposide in anti-CRPC development.
Keyphrases
- cell death
- cell cycle arrest
- cell proliferation
- signaling pathway
- pi k akt
- oxidative stress
- endothelial cells
- protein protein
- endoplasmic reticulum
- papillary thyroid
- binding protein
- induced apoptosis
- electronic health record
- squamous cell carcinoma
- fluorescent probe
- big data
- risk assessment
- pluripotent stem cells
- data analysis
- living cells
- lymph node metastasis
- quantum dots