Bioactive Peptides from Lupin ( Lupinus angustifolius ) Prevent the Early Stages of Atherosclerosis in Western Diet-Fed ApoE -/- Mice.
Guillermo Santos-SánchezIvan Cruz-ChamorroAna Isabel Álvarez-RíosNuria Álvarez-SánchezBeatriz Rodríguez-OrtizAna Isabel Álvarez-LópezMaría-Soledad Fernández-PachónJusto PedrocheFrancisco MillánMaría Del Carmen Millán-LinaresPatricia Judith LardoneIgnacio BejaranoAntonio Carrillo-VicoPublished in: Journal of agricultural and food chemistry (2022)
We have previously reported the in vitro hypocholesterolemic, anti-inflammatory, and antioxidant effects of Alcalase-generated lupin protein hydrolysate (LPH). Given that lipoprotein deposition, oxidative stress, and inflammation are the main components of atherogenesis, we characterized the LPH composition, in silico identified LPH-peptides with activities related to atherosclerosis, and evaluated the in vivo LPH effects on atherosclerosis risk factors in a mouse model of atherosclerosis. After 15 min of Alcalase hydrolysis, peptides smaller than 8 kDa were obtained, and 259 peptides out of 278 peptides found showed biological activities related to atherosclerosis risk factors. Furthermore, LPH administration for 12 weeks reduced the plasma lipids, as well as the cardiovascular and atherogenic risk indexes. LPH also increased the total antioxidant capacity, decreased endothelial permeability, inflammatory response, and atherogenic markers. Therefore, this study describes for the first time that LPH prevents the early stages of atherosclerosis.
Keyphrases
- oxidative stress
- cardiovascular disease
- risk factors
- amino acid
- inflammatory response
- mouse model
- anti inflammatory
- endothelial cells
- physical activity
- dna damage
- high fat diet
- fatty acid
- type diabetes
- toll like receptor
- south africa
- molecular docking
- signaling pathway
- protein protein
- binding protein
- lps induced
- heat shock
- wild type