Ultrastructural and immunohistochemical evaluation of hyperplastic soft tissues surrounding dental implants in fibular jaws.
Kezia Rachellea MustakimMi Young EoMi Hyun SeoHyeong-Cheol YangMin-Keun KimHoon MyoungSoung Min KimPublished in: Scientific reports (2024)
In reconstructive surgery, complications post-fibula free flap (FFF) reconstruction, notably peri-implant hyperplasia, are significant yet understudied. This study analyzed peri-implant hyperplastic tissue surrounding FFF, alongside peri-implantitis and foreign body granulation (FBG) tissues from patients treated at the Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital. Using light microscopy, pseudoepitheliomatous hyperplasia, anucleate and pyknotic prickle cells, and excessive collagen deposition were observed in FFF hyperplastic tissue. Ultrastructural analyses revealed abnormal structures, including hemidesmosome dilation, bacterial invasion, and endoplasmic reticulum (ER) swelling. In immunohistochemical analysis, unfolded protein-response markers ATF6, PERK, XBP1, inflammatory marker NFκB, necroptosis marker MLKL, apoptosis marker GADD153, autophagy marker LC3, epithelial-mesenchymal transition, and angiogenesis markers were expressed variably in hyperplastic tissue surrounding FFF implants, peri-implantitis, and FBG tissues. NFκB expression was higher in peri-implantitis and FBG tissues compared to hyperplastic tissue surrounding FFF implants. PERK expression exceeded XBP1 significantly in FFF hyperplastic tissue, while expression levels of PERK, XBP1, and ATF6 were not significantly different in peri-implantitis and FBG tissues. These findings provide valuable insights into the interconnected roles of ER stress, necroptosis, apoptosis, and angiogenesis in the pathogenesis of oral pathologies, offering a foundation for innovative strategies in dental implant rehabilitation management and prevention.
Keyphrases
- endoplasmic reticulum stress
- endoplasmic reticulum
- induced apoptosis
- oxidative stress
- gene expression
- signaling pathway
- poor prognosis
- epithelial mesenchymal transition
- cell cycle arrest
- minimally invasive
- cell death
- endothelial cells
- healthcare
- high resolution
- binding protein
- lps induced
- soft tissue
- cell proliferation
- body mass index
- mass spectrometry
- inflammatory response
- risk factors
- wound healing
- oral health
- long non coding rna
- weight gain
- amino acid
- physical activity
- breast cancer cells
- estrogen receptor
- simultaneous determination
- acute coronary syndrome
- weight loss
- high resolution mass spectrometry