Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology.
Ying YanQi ZhouXiaoqian RanQingyun LuCuishan ZhangMingyou PengLei TangRong-Can LuoYing-Tong DiXiaojiang HaoPublished in: International journal of molecular sciences (2023)
Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1 , 2 , 4 - 8 ). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identification of compounds 3 , 9 , and 10 as the previously published euphpepluones G, K, and L, respectively. All compounds were evaluated for their bioactivity with flow cytometry in assays of autophagic flux in HM Cherry-GFP-LC3 (human microglia cells stably expressing the tandem monomeric mCherry-GFP-tagged LC3) cells. Euphpepluone K ( 9 ) significantly activated autophagic flux, an effect that was verified with confocal analysis. Moreover, cellular assays showed that euphpepluone K ( 9 ) induced autophagy and inhibited Tau pathology.
Keyphrases
- cell death
- cell cycle arrest
- induced apoptosis
- endoplasmic reticulum stress
- flow cytometry
- signaling pathway
- oxidative stress
- high resolution
- endothelial cells
- high throughput
- cerebrospinal fluid
- high glucose
- inflammatory response
- mass spectrometry
- simultaneous determination
- randomized controlled trial
- spinal cord injury
- liquid chromatography
- spinal cord
- neuropathic pain
- optical coherence tomography
- pet ct
- wild type
- dual energy