Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods.

Tehmina BharuchaOnanong SengvilaipaseuthManivanh VongsouvathMalavanh VongsouvathViengmon DavongPhonepasith PanyanouvongGéraldine PiorkowskiJeremy A GarsonPaul N NewtonXavier de LamballerieAudrey Dubot-Pérès

Published in: PloS one (2018)

We succeeded in developing a JEV specific RT-qPCR assay with at least 1 log10 improved sensitivity as compared to existing assays. Further evaluation is required, field-testing the assay in a larger group of patients.

Keyphrases

high throughput
end stage renal disease
ejection fraction
newly diagnosed
chronic kidney disease
randomized controlled trial
patient reported outcomes
quantum dots
real time pcr
nucleic acid