Silver nanoparticles stimulate osteogenesis of human mesenchymal stem cells through activation of autophagy.
Wei HeYudong ZhengQingling FengTarek A ElkhoolyXujie LiuXing YangYansen WangYajie XiePublished in: Nanomedicine (London, England) (2020)
Aim: Previously, different results have been achieved regarding effects of silver nanoparticles (Ag NPs) on osteogenesis of stem cells and the mechanisms have not been disclosed yet, which are quite important for potential application of Ag NPs in bone reconstruction. Materials & methods: Effects of Ag NPs on osteogenesis of human mesenchymal stem cells (hMSCs) with underlying mechanisms were investigated. Results: Ag NPs at 2.5 and 5 μg/ml increased osteogenic proteins expression and mineralization of hMSCs. Meanwhile, autophagy was activated by Ag NPs and it could be inhibited by 3-methyladenine. Furthermore, osteogenesis induced by Ag NPs could also be reversed by 3-methyladenine. Conclusion: These findings suggest that autophagy is involved in stimulating osteogenesis of hMSCs induced by Ag NPs.
Keyphrases
- silver nanoparticles
- mesenchymal stem cells
- quantum dots
- stem cells
- highly efficient
- oxide nanoparticles
- visible light
- endothelial cells
- cell death
- bone regeneration
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- bone marrow
- umbilical cord
- poor prognosis
- cell therapy
- risk assessment
- body composition
- bone mineral density
- mass spectrometry
- single molecule
- bone loss