Modulation of inflammation by anti-TNF α mAb-dendrimer nanoparticles loaded in tyramine-modified gellan gum hydrogels in a cartilage-on-a-chip model.
I M OliveiraM R CarvalhoD C FernandesCatarina M AbreuFátima Raquel MaiaH PereiraDavid CaballeroS C KunduR L ReisJoaquim Miguel OliveiraPublished in: Journal of materials chemistry. B (2021)
Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disease characterized by joint inflammation. Since the inflammatory condition plays an important role in the disease process, it is important to develop and test new therapeutic approaches that specifically target and treat joint inflammation. In this study, a human 3D inflammatory cartilage-on-a-chip model was established to test the therapeutic efficacy of anti-TNFα mAb-CS/PAMAM dendrimer NPs loaded-Tyramine-Gellan Gum in the treatment of inflammation. The results showed that the proposed therapeutic approach applied to the human monocyte cell line (THP-1) and human chondrogenic primary cells (hCH) cell-based inflammation system revealed an anti-inflammatory capacity that increased over 14 days. It was also possible to observe that Coll type II was highly expressed by inflamed hCH upon the culture with anti-TNF α mAb-CS/PAMAM dendrimer NPs, indicating that the hCH cells were able maintain their biological function. The developed preclinical model allowed us to provide more robust data on the potential therapeutic effect of anti-TNF α mAb-CS/PAMAM dendrimer NPs loaded-Ty-GG hydrogel in a physiologically relevant model.
Keyphrases
- rheumatoid arthritis
- oxidative stress
- induced apoptosis
- endothelial cells
- drug delivery
- disease activity
- wound healing
- induced pluripotent stem cells
- pluripotent stem cells
- cell cycle arrest
- ankylosing spondylitis
- monoclonal antibody
- anti inflammatory
- single cell
- interstitial lung disease
- stem cells
- cell therapy
- extracellular matrix
- mesenchymal stem cells
- machine learning
- circulating tumor cells
- signaling pathway
- systemic sclerosis
- deep learning
- drug induced
- cell death
- smoking cessation
- artificial intelligence
- drug release