Hepatoprotective Effect of Polyphenol-Enriched Fraction from Folium Microcos on Oxidative Stress and Apoptosis in Acetaminophen-Induced Liver Injury in Mice.
Hongtan WuGang ZhangLisen HuangHaiyue PangNa ZhangYupei ChenGuey-Horng WangPublished in: Oxidative medicine and cellular longevity (2017)
Folium Microcos (FM), the leaves of Microcos paniculata L., shows various biological functions including antioxidant activity and α-glucosidase inhibitory effect. However, its therapeutic potential in acute liver injury is still unknown. This study investigated the hepatoprotective effect and underlying mechanisms of the polyphenol-enriched fraction (FMF) from Folium Microcos. FMF exhibited strong free radical scavenging activities and prevented HepG2/Hepa1-6 cells from hydrogen peroxide- (H2O2-) induced ROS production and apoptosis in vitro. Antioxidant activity and cytoprotective effects were further verified by alleviating APAP-induced hepatotoxicity in mice. Western blot analysis revealed that FMF pretreatment significantly abrogated APAP-mediated phosphorylation of MAPKs, activation of proapoptotic protein caspase-3/9 and Bax, and restored expression of antiapoptotic protein Bcl2. APAP-intoxicated mice pretreated with FMF showed increased nuclear accumulation of nuclear factor erythroid 2-related factor (Nrf2) and elevated hepatic expression of its target genes, NAD(P)H:quinine oxidoreductase 1 (NQO1) and hemeoxygenase-1(HO-1). HPLC analysis revealed the four predominantly phenolic compounds present in FMF: narcissin, isorhamnetin-3-O-β-D-glucoside, isovitexin, and vitexin. Consequently, these findings indicate that FMF possesses a hepatoprotective effect against APAP-induced hepatotoxicity mainly through dual modification of ROS/MAPKs/apoptosis axis and Nrf2-mediated antioxidant response, which may be attributed to the strong antioxidant activity of phenolic components.
Keyphrases
- drug induced
- liver injury
- oxidative stress
- diabetic rats
- cell death
- hydrogen peroxide
- induced apoptosis
- dna damage
- nuclear factor
- endoplasmic reticulum stress
- high glucose
- poor prognosis
- binding protein
- nitric oxide
- ms ms
- endothelial cells
- single cell
- toll like receptor
- liver failure
- intensive care unit
- south africa
- heat shock
- long non coding rna
- metabolic syndrome
- anti inflammatory
- hepatitis b virus
- immune response
- signaling pathway
- insulin resistance
- adipose tissue
- simultaneous determination
- data analysis