Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes.
Bruna PaulsenSabrina PiechotaFerran BarrachinaAlexa GiovanniniSimone KatsKathryn S PottsGraham RockwellMaria MarchanteSamantha L EstevezAlexander D NoblettAlexandra B FigueroaCaroline AschenbergerDawn A KelkMarcy FortiShelby MarcinyshynKlaus WiemerMarta SanchezPedro BelchinJoseph A LeeErkan BuyukRick E SlifkinMerrick Pierson SmelaPatrick R J FortunaPranam ChatterjeeDavid H McCullohAlan B CoppermanDaniel Ordonez-PerezJoshua U KleinChristian C KrammePublished in: Journal of assisted reproduction and genetics (2024)
The OSC-IVM platform is a novel tool for rescue maturation of human oocytes, yielding oocytes with improved nuclear maturation and a closer transcriptomic resemblance to in vivo matured oocytes, indicating a potential enhancement in oocyte cytoplasmic maturation. These improvements on oocyte quality after OSC-IVM are possibly occurring through bidirectional crosstalk of cumulus-free oocytes and ovarian support cells.